Why does EzBioCloud yield different similarity values compared to BLAST or other services?

Why does EzBioCloud yield different similarity values compared to BLAST or other services?

Pairwise sequence similarity is the key criterion for microbial identification and as suggested by Kim et al. (2014), a species boundary is defined by a similarity value of 98.65% or greater. In other words, if two sequences show a similarity of 98.65% or less, they belong to different species. BLAST and other services may produce different similarities as they use different algorithms. However, we recommend avoiding BLAST for taxonomy-related sequence identity values. Learn more