Yes. EzBioCloud’s “Identify” engine automatically recognizes reverse-complement sequences. They are corrected before being fed to our identification pipeline. We save the corrected sequences in your account, instead of the original version.
Pairwise sequence similarity is the key criterion for microbial identification and as suggested by Kim et al. (2014), a species boundary is defined by a similarity value of 98.65% or greater. In other words, if two sequences show a similarity of 98.65% or less, they belong to different species. BLAST and other services may produce different similarities as they use different algorithms. However, we recommend avoiding BLAST for taxonomy-related sequence identity values. Learn more
For public users, up to 200 results are saved. If a new result is added, the oldest result will be deleted.
Up to ten identifications can be running simultaneously, but they need to be submitted one by one. The ‘Identify single sequence’ button allows the user to submit a single 16S rRNA gene sequence for identification, but the user can sequentially submit additional single sequences for identification, and the system will allow up to 10 identification jobs running simultaneously per user.
We plan to update our database every two months, starting in January 2017.